Cannot overwrite fcs files when re-load old data

[roelar]

Hello: I am trying a different annotation for the Rphenograph clustering in Shinyapp but when I try to save the new annotation files I don't see new fcs files or the updated version of the old fcs files. It seems to be a problem with finding the original files to overwrite. This is the message I get:

/var/folders/8l/1lyl6pp91gd4y13wgg1t33qs2c6gtc/T//RtmpEtgtl2/5eec6fa99cb99ee93af15196/0.RDataWarning in eval(expr, env) :
Path for original FCS files doesn't exist,
data cannnot be saved to new copies of FCS files.Please check path: /var/folders/8l/1lyl6pp91gd4y13wgg1t33qs2c6gtc/T//RtmpbMciCe/dd196aa577b154f77dc7e5c0
Writing results Done! Results are saved under path: ./

Both, csv and .RData are generated, but there is no new folder of analyzed fcs files. Could you please indicate what could I change to fix this? I tried using different working directory with the original fcs files in it but still not working.
Thank you very much!

[SamGG]

Hi,
To export FCS files, the original FCS are needed and should be located at the exact same location when you did your first analysis of this set of FCS. I think...
Best.