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scWGCNA

WARNING!!!!!!

scWGCNA is currently under construction! Everything is being overhauled and tons of new features are being added. You can check the dev branch, or check the fancy new website for a sneak peak. For now use the dev branch with caution because it is changing on a pretty much daily basis, but I am planning to have a stable release of the updated scWGCNA at the beginning of March.

scWGCNA is a bioinformatics workflow and an add-on to the R package WGCNA to perform weighted gene co-expression network analysis in single-cell or single-nucleus RNA-seq datasets. WGCNA was originally built for the analysis of bulk gene expression datasets, and the performance of vanilla WGCNA on single-cell data is limited due to the inherent sparsity of scRNA-seq data. To account for this, scWGCNA has a function to aggregate transcriptionally similar cells into pseudo-bulk metacells before running the WGCNA pipeline. Furthermore, WGCNA is a well established tool with many different options and parameters, so we recommend trying different options in network construction that are best suited to your dataset.

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WGCNA adapted for single cell RNA-seq

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