dgeAnalysis

This R package contains the R-Shiny application v1.5.2 developed to perform differential gene expression analysis.

• dgeAnalysis manual: https://github.com/LUMC/dgeAnalysis/blob/master/MANUAL.pdf

Installing

1. Install the "devtools" package (if not already installed):

install.packages("devtools")

2. Install the "dgeAnalysis" package:

library("devtools")
devtools::install_github("LUMC/dgeAnalysis")

3. Launch the application:

library("dgeAnalysis")
dgeAnalysis::startApp()

Quick start

1. Start the application
2. Go to "Data upload"
3. Upload files: (both CSV and TSV is accepted)
   • Samplesheet (metadata), Contains information about each sample.
   • Count data, Contains the read alignment results (read counts per feature).
   • Annotation, Contains more information about genes (location, length, etc.).
4. Set a comparison
5. Run the analysis
6. View the results

Note: In the folder "exampleData" there is a .zip file, containing three data files. These example files can be used to e.g. check if the application is working and/or your data has the correct format.

Requirements

R version: 4.1+

Linux install libraries:

sudo apt-get update && apt-get install \
   build-essential \
   gdebi-core \
   pandoc \
   pandoc-citeproc \
   libcairo2-dev \
   libjpeg-dev \
   libxt-dev \
   libssl-dev \
   libcurl4-gnutls-dev \
   libxml2-dev \
   libcurl4-openssl-dev \
   -y

Windows install RTools and pandoc:
R 4.0 and up:
  https://cran.r-project.org/bin/windows/Rtools
Pandoc:
  https://pandoc.org/installing.html

Fix install problems

If there are problems installing the application, it might be worth to try installing specific packages manually:

## Shiny environment
if (!require("shiny")) install.packages("shiny")
if (!require("shinydashboard")) install.packages("shinydashboard")
if (!require("shinyWidgets")) install.packages("shinyWidgets")
if (!require("shinycssloaders")) install.packages("shinycssloaders")
if (!require("shinyjs")) install.packages("shinyjs")

## Differential expression analysis
if (!require("BiocManager")) install.packages("BiocManager")
if (!require("knitr")) install.packages("knitr")
if (!require("SummarizedExperiment")) BiocManager::install("SummarizedExperiment")
if (!require("edgeR")) BiocManager::install("edgeR")
if (!require("limma")) BiocManager::install("limma")
if (!require("DESeq2")) BiocManager::install("DESeq2")
if (!require("tidyr")) install.packages("tidyr")
if (!require("scales")) install.packages("scales")
if (!require("broom")) install.packages("broom")
if (!require("plotly")) install.packages("plotly")
if (!require("Rtsne")) install.packages("Rtsne")
if (!require("rmarkdown")) install.packages("rmarkdown")

## Enrichment analysis
if (!require("gprofiler2")) install.packages("gprofiler2")
if (!require("igraph")) install.packages("igraph")