Contains data and code necessary to reproduce analyses that appear in “Sexual dimorphism and the multi-omic response to exercise training in rat subcutaneous white adipose tissue”, published in Nature Metabolism (https://doi.org/10.1038/s42255-023-00959-9).
- Differential analysis of transcriptomics, proteomics, phosphoproteomics, and metabolomics subcutaneous white adipose tissue -omics datasets.
- Enrichment analyses (FGSEA or KSEA) for each -ome.
- Weighted Gene Co-expression Network Analysis for all -omes excluding proteomics.
- Over-representation analysis (ORA) performed on WGCNA modules/clusters.
The data-raw/ folder contains code to reproduce all analyses. Most R script names match the objects they create, though some scripts create multiple objects (such as WATSC_DA.R), and so are given more general names. Code to reproduce figures will likely be added as vignettes at a later date.
devtools::install_github("MoTrPAC/MotrpacRatTraining6moWATData")The MotrpacRatTraining6moWAT R package contains helper functions for analysis and visualization of the data contained in MotrpacRatTraining6moWATData.
For questions, bug reporting, and data requests for this package, please submit a new issue and include as many details as possible.
If the concern is related to functions provided in the MotrpacRatTraining6moWAT package, please submit an issue here instead.
MoTrPAC is supported by the National Institutes of Health (NIH) Common Fund through cooperative agreements managed by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institute of Arthritis and Musculoskeletal Diseases (NIAMS), and National Institute on Aging (NIA).
Specifically, the MoTrPAC Study is supported by NIH grants U24OD026629 (Bioinformatics Center), U24DK112349, U24DK112342, U24DK112340, U24DK112341, U24DK112326, U24DK112331, U24DK112348 (Chemical Analysis Sites), U01AR071133, U01AR071130, U01AR071124, U01AR071128, U01AR071150, U01AR071160, U01AR071158 (Clinical Centers), U24AR071113 (Consortium Coordinating Center), U01AG055133, U01AG055137 and U01AG055135 (PASS/Animal Sites).
Recipients and their Agents agree that in publications using any data from MoTrPAC public-use data sets they will acknowledge MoTrPAC as the source of data, including the version number of the data sets used, e.g.:
- Data used in the preparation of this article were obtained from the Molecular Transducers of Physical Activity Consortium (MoTrPAC) database, which is available for public access at motrpac-data.org. Specific datasets used are [version numbers].
- Data used in the preparation of this article were obtained from the Molecular Transducers of Physical Activity Consortium (MoTrPAC) MotrpacRatTraining6moWATData R package [version number].