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Astrocyte CRISPRi Resource

pipeline

Launch site.

This website allows the exploration of CRISPR interference (CRISPRi) data on enhancers in normal human astrocytes (NHAs), from Green et al. 2024. For each enhancer-gene pair tested in the screen, the following data is available:

  • Statistical significance and effect size (Hit, log2FC, P, FDR), displayed in a searchable table and displayed on the corresponding Volcano plot.
    • search the table by gene name or hg38 genome coordinates.
    • to download the filtered data click on the CSV button.
  • ATAC-seq, TT-seq, and Deep-learning-based functional variant annotation, displayed as a UCSC genome browser track collection.
    • select an enhancer-gene pair in the table OR plot and click the UCSC button to navigate to the enhancer position.
    • select an enhancer-gene pair and click the prediction button to navigate to the enhancer's DeepSEA (beluga) heatmap viewer.

Code for the heatmap viewer can be found here.