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Retrying -r on pod5 convert fast5
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Msoliman00 committed Aug 8, 2023
1 parent af9bd26 commit 9d8c335
Showing 1 changed file with 2 additions and 2 deletions.
4 changes: 2 additions & 2 deletions dorado_basecall.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -53,15 +53,15 @@ task basecall {
mkdir fast5s
tar zxvf ~{fast5_archive} -C fast5s
# Convert FAST5 into POD5
pod5 convert fast5 ./fast5s/*.fast5 -o pod5s/reads.pod5 --threads 12
pod5 convert fast5 -r fast5s -o pod5s/reads.pod5 --threads 12
fi

if [[ "$filetype" == *"Zip archive data"* ]]; then
echo "FAST5s appear to be compressed with zip. Decompressing..."
mkdir fast5s
unzip ~{fast5_archive} -d fast5s
# Convert FAST5 into POD5
pod5 convert fast5 ./fast5s/*.fast5 -o /pod5s/reads.pod5 --threads 12
pod5 convert fast5 -r fast5s -o pod5s/reads.pod5 --threads 12
fi

# Simplex call with --emit-moves and --modified-bases
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