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Update README.md
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abartlett004 authored Oct 1, 2024
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## Run data with nf-core rnaseq

- Make sure you have access to our [Seqera WorkSpace](https://cloud.seqera.io/orgs/HBC/workspaces/core_production/launchpad)
- Transfer data to HCBC S3: Ask Alex/Lorena. Files will be at our S3 bucket `input/rawdata` folder
- Prepare the CSV file according this [instructions](https://nf-co.re/rnaseq/3.14.0/docs/usage#multiple-runs-of-the-same-sample). File should look like this:
- Transfer data to HCBC S3: Ask Alex/Lorena. Files will be at our S3 bucket `input` folder
- Prepare the CSV file according these [instructions](https://nf-co.re/rnaseq/3.14.0/docs/usage#multiple-runs-of-the-same-sample). File should look like this:

```csv
sample,fastq_1,fastq_2,strandedness
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- Upload file to our `Datasets` in Seqera using the name of the project but starting with `rnaseq-pi_lastname-hbc_code`
- Go to `Launchpad`, select `nf-core_rnaseq` pipeline, and select the previous created `Datasets` in the `input` parameter after clicking in `Browser`
- Select an output directory with the same name used for the `Dataset` inside the `results` folder in S3
- When pipeline is down, data will be copied to our on-premise HPC in the scratch system under `scratch/groups/hsph/hbc/bcbio/` folder
- When pipeline is done, data will be copied to our on-premise HPC in the scratch system under `scratch/groups/hsph/hbc/bcbio/` folder

## Downstream analysis

Please, modify `information.R` with the right information. You can use this file with any other Rmd to include the project/analysis information.
Modify `information.R` with the right information. You can use this file with any other Rmd to include the project/analysis information.

### QC

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### DE

`DE/DEG.Rmd` is a template for two groups comparison. `params_de.R` has the information of the input files to load. You can point to `bcbio` or `nf-core/rnaseq` output files.
`DE/DEG.Rmd` is a template for comparison between two groups. `params_de.R` has the information for the input files to load. You can point to `bcbio` or `nf-core/rnaseq` output files.

On the `YAML` header file of the `Rmd` you can specify some parameters or just set them up in the first chunk of code of the template. This template has examples of:

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