MoP2- DSL2 version of Master of Pores

Inspired by Metallica's Master Of Puppets

Install

Please install nextflow and singularity or docker before.

Then download the repo:

git clone --depth 1 --recurse-submodules [email protected]:biocorecrg/MOP2.git

or

git clone --depth 1 --recurse-submodules https://github.com/biocorecrg/MOP2.git

You can use INSTALL.sh to download the version 3.4.5 of guppy or you can replace it with the version you prefer. Please consider that the support of VBZ compression of fast5 started with version 3.4.X.

cd MOP2; sh INSTALL.sh 3.4.5

Testing

You can replace -with-singularity with -with-docker if you want to use the docker engine.

cd mop_preprocess
nextflow run mop_preprocess.nf -with-singularity -bg -profile local > log

Reference

If you use this tool, please cite our papers:

"Nanopore Direct RNA Sequencing Data Processing and Analysis Using MasterOfPores" Cozzuto L, Delgado-Tejedor A, Hermoso Pulido T, Novoa EM, Ponomarenko J. N. Methods Mol Biol. 2023;2624:185-205. doi: 10.1007/978-1-0716-2962-8_13.

"MasterOfPores: A Workflow for the Analysis of Oxford Nanopore Direct RNA Sequencing Datasets" Luca Cozzuto, Huanle Liu, Leszek P. Pryszcz, Toni Hermoso Pulido, Anna Delgado-Tejedor, Julia Ponomarenko, Eva Maria Novoa. Front. Genet., 17 March 2020. https://doi.org/10.3389/fgene.2020.00211

Documentation

The documentation is available at https://biocorecrg.github.io/MOP2/docs/