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def run_big_stitcher(): | ||
return |
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run("Load TileConfiguration from File...", | ||
"browse=D:/TiledDataset/2.5x_tile/2.5x_tile_igor_rightonly_Mag2.5x_ch488_ch561_ch647_bdv3.xml " + | ||
"select=D:/TiledDataset/2.5x_tile//2.5x_tile_igor_rightonly_Mag2.5x_ch488_ch561_ch647_bdv3.xml " + | ||
"tileconfiguration=D:/TiledDataset/2.5x_tile//2.5x_tile_igor_rightonly_Mag2.5x_ch488_ch561_ch647_bdv_tile_config.txt " + | ||
"use_pixel_units keep_metadata_rotation"); | ||
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run("Calculate pairwise shifts ...", | ||
"select=D:/TiledDataset/2.5x_tile//2.5x_tile_igor_rightonly_Mag2.5x_ch488_ch561_ch647_bdv3.xml " + | ||
"process_angle=[All angles] process_channel=[All channels] process_illumination=[All illuminations] " + | ||
"process_tile=[All tiles] process_timepoint=[All Timepoints] " + | ||
"method=[Phase Correlation] channels=[Average Channels] " + | ||
"downsample_in_x=4 downsample_in_y=4 downsample_in_z=4"); | ||
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run("Optimize globally and apply shifts ...", | ||
"select=D:/TiledDataset/2.5x_tile//2.5x_tile_igor_rightonly_Mag2.5x_ch488_ch561_ch647_bdv3.xml " + | ||
"process_angle=[All angles] process_channel=[All channels] process_illumination=[All illuminations] " + | ||
"process_tile=[All tiles] process_timepoint=[All Timepoints] relative=2.500 absolute=3.500 " + | ||
"global_optimization_strategy=[Two-Round using Metadata to align unconnected Tiles and iterative dropping of bad links] " + | ||
"fix_group_0-0,"); |