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docs: do not activate python env #461

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May 31, 2024
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19 changes: 8 additions & 11 deletions docs/run_on_restricted_system_env.md
Original file line number Diff line number Diff line change
Expand Up @@ -15,11 +15,8 @@ Fetch the pipeline and install requirements
TAG_OR_BRANCH="vX.Y.X"

# Clone selected version
git clone --branch ${VERSION} https://github.com/genomic-medicine-sweden/Twist_Solid.git
git clone --branch ${TAG_OR_BRANCH} https://github.com/genomic-medicine-sweden/Twist_Solid.git
cd Twist_Solid
# Python 3.8 or newer
python3 -m venv venv && source venv/bin/activate
pip install -r requirements.txt
```

## Environment
Expand Down Expand Up @@ -50,12 +47,12 @@ hydra-genetics prepare-environment create-singularity-files -c config/config.yam

```bash
# NextSeq
hydra-genetics --debug references download -o design_and_ref_files -v config/references/design_files.hg19.yaml -v config/references/nextseq.hg19.pon.yaml -v config/references/references.hg19.yaml
hydra-genetics --debug references download -o design_and_ref_files -v config/references/design_files.hg19.yaml -v config/references/nextseq.hg19.pon.yaml -v config/references/references.hg19.yaml

#NovaSeq, not all files are prepare for novaseq
hydra-genetics references download -o design_and_ref_files -v config/references/design_files.hg19.yaml -v config/references/novaseq.hg19.pon.yaml -v config/references/references.hg19.yaml
#NovaSeq, not all files are prepare for novaseq
hydra-genetics references download -o design_and_ref_files -v config/references/design_files.hg19.yaml -v config/references/novaseq.hg19.pon.yaml -v config/references/references.hg19.yaml

# Compress data
# Compress data
tar -czvf design_and_ref_files.tar.gz design_and_ref_files
```

Expand All @@ -66,7 +63,7 @@ tar -czvf design_and_ref_files.tar.gz design_and_ref_files
The following file/folders have been created and need to be moved to your server:

1. file: design_and_ref_files.tar.gz
2. file Twist_Solid_{TAG_OR_BRANCH}.tar.gz
2. file: Twist_Solid_{TAG_OR_BRANCH}.tar.gz
3. folder: singularity_cache

---
Expand All @@ -81,7 +78,7 @@ The following file/folders have been created and need to be moved to your server
# Extract tar.
TAG_OR_BRANCH="vX.Y.X"
tar -xvf Twist_Solid_${TAG_OR_BRANCH}.tar.gz
cd Twist_Solid_{TAG_OR_BRANCH}
cd Twist_Solid_${TAG_OR_BRANCH}
mkdir venv && tar xvf env.tar.gz -C venv/
source venv/bin/activate

Expand Down Expand Up @@ -142,7 +139,7 @@ Copy a profile and modify it to match your system, ex```Twist_Solid_${TAG_OR_BRA
```yaml
# Found at Twist_Solid_{TAG_OR_BRANCH}/snakemake-wrappers, use absolute_path with 'git+file:/'
wrapper-prefix="PATH_TO_WRAPPERS"
# ex: wrapper-prefix="git+file://proj/sens2022566/nobackup/patriksm/Twist_Solid_add-{TAG_OR_BRANCH}/snakemake-wrappers/"
# ex: wrapper-prefix: "git+file://proj/sens2022566/nobackup/patriksm/Twist_Solid_add-{TAG_OR_BRANCH}/snakemake-wrappers/"

# Update account info, change ADD_YOUR_ACCOUNT to your bianca project id
drmaa: " -A ADD_YOUR_ACCOUNT -N 1-1 -t {resources.time} -n {resources.threads} --mem={resources.mem_mb} --mem-per-cpu={resources.mem_per_cpu} --mem-per-cpu={resources.mem_per_cpu} --partition={resources.partition} -J {rule} -e slurm_out/{rule}_%j.err -o slurm_out/{rule}_%j.out"
Expand Down
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