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297 add nanopore test data (#337)
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* add ONT test data and samplelist for ONT

* force adding long-reads test data
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Emkago authored Aug 16, 2024
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3 changes: 3 additions & 0 deletions CHANGELOG.md
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Expand Up @@ -9,6 +9,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0

### Added

- Added long-read test data (S. aureus)
- Added `samplelist_nanopore.csv` for running long-read test data
- Added location of documentation to `README`
- Added `cdmDir` to config

### Fixed
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4 changes: 2 additions & 2 deletions README.md
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Expand Up @@ -29,12 +29,12 @@ See the documentation for installation instructions.

## Documentation

The documentation is abailable for the latest stable release.
The documentation `docs/source` is available for the latest stable release.

## Contributing

Contributions to the pipeline is more than welcome. Please use the [CONTRIBUTING](CONTRIBUTING.md) file for details.

## License

Jasen is released under the GPLv3 license.
Jasen is released under the GPLv3 license.
2 changes: 2 additions & 0 deletions assets/test_data/samplelist_nanopore.csv
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id,platform,sequencing_run,read1
test_mecA,nanopore,20231017_1501_MN39800_ARA658_5ab3fe12,PATH_TO_JASEN/assets/test_data/sequencing_data/saureus_nanopore_1k/saureus_nanopore_1k.fastq.gz
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5 changes: 3 additions & 2 deletions docs/source/install.md
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Expand Up @@ -67,9 +67,10 @@ When analysing Nanopore data:
* Edit the `ext.args` for Medaka: specify the model with flag `-m`. Currently it is set to `r941_min_sup_g507`, but one should always set it based on how the data was produced. More about choosing the right model can be found [here](https://github.com/nanoporetech/medaka#models).

### Test data
Source: `assets/test_data/samplelist.csv`
Source: `assets/test_data/samplelist*.csv`

* Edit the read1 and read2 columns in `assets/test_data/samplelist.csv`
* For short reads produced with Illumina or IonTorrent technology, edit the read1 and read2 columns in `assets/test_data/samplelist.csv`
* For long reads produced with ONT technology, edit the read1 column in `assets/test_data/samplelist_nanopore.csv`

## Setting up temp directories

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