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# Nup133
# SEA Complex (UNDER CONSTRUCTION)

These scripts demonstrate the use of [IMP](http://salilab.org/imp),
[MODELLER](http://salilab.org/modeller/),
[FoXS](http://salilab.org/foxs/), and
[PMI](https://github.com/salilab/pmi),
[FoXS](http://salilab.org/foxs), and
[Minimal Ensemble Search](http://bl1231.als.lbl.gov/saxs_protocols/mes.php) in the modeling of the
Nup133 in the Nuclear Pore Complex (NPC). First, MODELLER is used to generate
SEA complex. First, MODELLER is used to generate
structures for the individual components in the Nup133. Then, IMP
is used to fit these components together into the electron microscopy density
map of Nup133.

A full description of the scripts can be found in
[Integrative structure-function mapping of the nucleoporin Nup133 suggests a conserved mechanism for membrane anchoring of the nuclear pore complex](http://mcponline.org/content/early/2014/08/19/mcp.M114.040915).
[Molecular architecture and function of the SEA complex, a modulator of the TORC1 pathway](http://mcponline.org/content/early/2014/07/29/mcp.M114.039388).

## Steps (UNDER CONSTRUCTION)

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## Information

_Author(s)_: Seung Joong Kim
_Author(s)_: Seung Joong Kim, Riccardo Pellarin, Peter Cimermancic3

_Date_: September 2nd, 2014

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_Parallelizeable_: No

_Publications_:
- Seung Joong Kim*, Javier Fernandez-Martinez*, Parthasarathy Sampathkumar*, Anne Martel, Tsutomu Matsui, Hiro Tsuruta, Thomas Weiss, Yi Shi, Ane Markina-Inarrairaegui, Jeffery B. Bonanno, J. Michael Sauder, Stephen K. Burley, Brian T. Chait, Steven C. Almo, Michael P. Rout, and Andrej Sali, [Integrative structure-function mapping of the nucleoporin Nup133 suggests a conserved mechanism for membrane anchoring of the nuclear pore complex](http://mcponline.org/content/early/2014/08/19/mcp.M114.040915), Molecular & Cellular Proteomics, 2014, mcp.M114.040915.
- Romain Algret, Javier Fernandez-Martinez, Yi Shi, Seung Joong Kim, Riccardo Pellarin, Peter Cimermancic, Emilie Cochet, Andrej Sali, Brian T. Chait, Michael P. Rout, and Svetlana Dokudovskaya, [Molecular architecture and function of the SEA complex, a modulator of the TORC1 pathway](http://mcponline.org/content/early/2014/07/29/mcp.M114.039388), Molecular & Cellular Proteomics, 2014, mcp.M114.039388.

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