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Fixing README.md
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Jennifer Lu committed Sep 10, 2018
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Expand Up @@ -120,10 +120,12 @@ The kmer distribution file is generated using the following command line:
## Step 2: Run Kraken 1.0 or Kraken 2.0 AND Generate a report file

Kraken 1.0 requires a 2-step process to generate the report file needed by Bracken

kraken --db ${KRAKEN_DB} --threads ${THREADS} ${SAMPLE}.fq > ${SAMPLE}.kraken
kraken-report --db ${KRAKEN_DB} ${SAMPLE}.kraken > ${SAMPLE}.kreport

Kraken 2.0 requires the addition of the --report flag

kraken2 --db ${KRAKEN_DB} --threads ${THREADS} --report ${SAMPLE}.kreport ${SAMPLE}.fq > ${SAMPLE}.kraken

## Step 3: Run Bracken for Abundance Estimation
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