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One way I'm looking at resolving this issue is by using deepvariant directly, instead of running deepvariant through clara parabricks. deepvariant looks to have a --sample_name flag (where clara parabricks doesn't expose this flag). So far I've been hamstring into modifying the @RG tags in the in the aligned BAM with samtools addreplacerg or during alignment with the -R flag in minimap2. But I think running deepvariant directly and using it's --sample_name flag will allow me to avoid this bug as well as any modification to the @RG tags, which is probably ideal. This potential fix could also resolve the related issue #8.
Pipeface version: v0.1.1
Nextflow version: 24.04.1
Error message in nextflow:
But the error comes upstream from minimap2
Error message in Minimap2 log:
I think this error comes from utilising the
-R
flag in Minimap2, when the input uBAM doesn't have read group info (@RG
tag)The text was updated successfully, but these errors were encountered: