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Data processing software for dSTORM super-resolution microscopy
lfiaschi/simple-STORM
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/************************************************************************/ /* */ /* ANALYSIS OF STORM DATA */ /* */ /* Copyright 2010-2011 by Joachim Schleicher and Ullrich Koethe */ /* */ /* Please direct questions, bug reports, and contributions to */ /* [email protected] */ /************************************************************************/ dSTORM microscopy can improve the resolution of conventional fluorescent microscopy by one order of magnitude. The data analysis software has to localize many thousand single molecule point-spread-functions to reconstruct an image of the underlying structure. Content of this archive: storm/ sources for single-molecule-localization and command-line utility to analyze .sif and .hdf5 input data gui/ Qt-based graphical-user-interface (GUI) for selection of input files and appropriate parameter(s) python/ helper scripts to analyze and display the coordinate lists generated by the utilities above colorcomposer/ multi-channel measurements have to be aligned due to chromatic aberrations. Tetraspeck-Beads are detected automatically so that the images can be registrated easily. See INSTALL for instructions on how to build and install storm or storm-gui More algorithmic details are available in the diploma thesis at http://hci.iwr.uni-heidelberg.de/Staff/jschleic/
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Data processing software for dSTORM super-resolution microscopy
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