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Wrong results without error if saved_trimmed = true #1481

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panzhang666666 opened this issue Dec 23, 2024 · 0 comments
Open

Wrong results without error if saved_trimmed = true #1481

panzhang666666 opened this issue Dec 23, 2024 · 0 comments
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@panzhang666666
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panzhang666666 commented Dec 23, 2024

Description of the bug

Hi, I find a wired thing that if saved_trimmed = true used when I using fastp as trimmer, the mapped reads reduced a lot. Take OMICS_356_21 as an example, the M reads goes from 100.6M to 64.7M if I set saved_trimmed to true without any other parameter change. And both 2 pipeline finished successfully with " [main] INFO nextflow.Nextflow - -[nf-core/rnaseq] Pipeline completed successfully -". Does anyone know why?

Many thanksImage
Image

Command used and terminal output

Read trimming options
trimmer : fastp
extra_fastp_args : --length_required 50 --cut_by_quality3 3 --cut_by_quality5 3 --cut_window_size 4 --cut_mean_quality 15

Alignment options
min_mapped_reads : 5

Quality Control
rseqc_modules : inner_distance,read_distribution

Process skipping options
skip_umi_extract : true
skip_pseudo_alignment: true
skip_markduplicates : true
skip_bigwig : true
skip_stringtie : true
skip_dupradar : true
skip_qualimap : true
skip_biotype_qc : true

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System information

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@panzhang666666 panzhang666666 added the bug Something isn't working label Dec 23, 2024
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