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Revise truncated pseudo attributes #333

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merged 5 commits into from
Oct 8, 2024
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@oschwengers oschwengers commented Oct 8, 2024

Bakta and various standard output formats (Genbank, EMBL, GFF3) use slightly different terms and approaches how to declare truncated genes and pseudogenes.

In Bakta, a feature is declared as truncated if there is information from a downstream analysis tool, e.g. Pyrodigal, Infernal, etc.

Besides these, Bakta accepts true pseudogenes from tRNAscan-SE and from its own internal CDS workflow.

To strictly follow INSDC specs, for Genbank, EMBL and GFF3 output files (#330), Bakta now declares all truncated features as pseudo reflecting technical issues like sequencing and assembly errors on the one side, and true pseudogenes on the other side emerging from biological pseudogenization events like InDels and mutations.

Internally, Bakta uses truncated and pseudogene attributes to reflect the different states. In the human readable TSV output file (meant for a quick glimpse), Bakta adds feature product prefixes (pseudo), (truncated), (5' truncated) and (3' truncated)`.

@oschwengers oschwengers added the enhancement New feature or request label Oct 8, 2024
@oschwengers oschwengers added this to the v1.10.0 milestone Oct 8, 2024
@oschwengers oschwengers self-assigned this Oct 8, 2024
@oschwengers oschwengers merged commit 041fb94 into main Oct 8, 2024
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@oschwengers oschwengers deleted the revise-truncated-pseudo branch October 8, 2024 14:29
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