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I am utilizing the Seurat Object for analyzing the single cell data analysis in R, I am following the steps in Seurat to have a thorough understanding, however I see the analysis starts with creating the seurat object with barcodes.tsv, genes.tsv, matrix.mtx fiels from teh step #####Loading and reading the PBMC data#####
pbmc.data<-Read10X(data.dir="---/-----/----/hg19/"), i was thinking do teh single cell data analysis with barcode, genes and matrix files or the single cell RNA seq data is processed through the fastq files, if so, could you please add the .fastq files and the preprocessing steps in the data preprocessing also, could you please provide the information of raw data .fastq files.
Happy Coding
Thank you
Divya
The text was updated successfully, but these errors were encountered:
I am utilizing the Seurat Object for analyzing the single cell data analysis in R, I am following the steps in Seurat to have a thorough understanding, however I see the analysis starts with creating the seurat object with barcodes.tsv, genes.tsv, matrix.mtx fiels from teh step #####Loading and reading the PBMC data#####
pbmc.data<-Read10X(data.dir="---/-----/----/hg19/"), i was thinking do teh single cell data analysis with barcode, genes and matrix files or the single cell RNA seq data is processed through the fastq files, if so, could you please add the .fastq files and the preprocessing steps in the data preprocessing also, could you please provide the information of raw data .fastq files.
Happy Coding
Thank you
Divya
The text was updated successfully, but these errors were encountered: