Flow cytometry data analysis basic template

Description

This template performs some basic preprocessing, clustering and dimension reduction steps on flow cytometry data.

Steps

• Preprocessing: channel-specific logicle transformation
• Clustering using PhenoGraph
• Dimension Reduction using UMAP
• Marker enrichment score
• Cell population (cluster) proportions computation across samples
• Relevant data visualisations

Requirements

• Some FCS data imported using the FCS Tercen importer
• A Channel Annotation file as generated by the FCS importer