Behavioural Tracker Analysis for bright field full field images. (See branch v2-L1Worms for adjusted code for smaller, more transparent L1 worms)
Use virtural stack taken by Realtimetracker_09 or after bright field full field images (needs higer than imagej 1.44k)
- open virtual stack
- start BFT plugin
- click Setup and set threshold and roi size (min,max). this filed has to be hit return/enter key
- choose scale 10x 4x
- Go
- check if head and tail are detected correctly.
- If not, click swap botton to change all frame, or click the graph to swap each period, or click each slice image one by one.
- output data and curvature. the unit of angles is radian
- 20607 gave up to track coiling situation (touching head or tail to the body) could be detectable omegaturn by combination of reverse/deepbend/touching. just transferrig .jar file made by eclipse to plugin folder of imagej didn't work transfer /bin/*.class to plugin/BFT did work.