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locale/de/LC_MESSAGES/Glossary.po

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+# SOME DESCRIPTIVE TITLE.
+# Copyright (C) 2023
+# This file is distributed under the same license as the Python package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, 2023.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: Python \n"
+"Report-Msgid-Bugs-To: \n"
+"POT-Creation-Date: 2023-05-19 07:07-0400\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language: de\n"
+"Language-Team: de <[email protected]>\n"
+"Plural-Forms: nplurals=2; plural=(n != 1);\n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=utf-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+"Generated-By: Babel 2.12.1\n"
+
+#: ../../Glossary.md:1
+msgid "Glossary"
+msgstr ""
+
+#: ../../Glossary.md
+msgid "Blocking"
+msgstr ""
+
+#: ../../Glossary.md:5
+msgid ""
+"During the immunostaining procedure, it is important to minimize "
+"nonspecific binding of the primary or secondary antibodies. In most "
+"cases, this is achieved by blocking, which typically involves adding "
+"substances such as normal sera, gelatin, or albumin before immunostaining"
+" in order to \"occupy\" all the non-specific binding sites in the sample."
+msgstr ""
+
+#: ../../Glossary.md:3
+msgid "Deconvolution"
+msgstr ""
+
+#: ../../Glossary.md:8
+msgid ""
+"The process of computationally removing blur from microscopy images by "
+"using the known optical properties of the light path to \"reassign\" "
+"pixel intensity away from where it hit the camera and back onto the "
+"structure that emitted the light."
+msgstr ""
+
+#: ../../Glossary.md:6
+msgid "Ex-Vivo imaging"
+msgstr ""
+
+#: ../../Glossary.md:11
+#, python-format
+msgid ""
+"Refers to imaging performed on live animal tissue in an external "
+"controllable environment (e.g., tissue explant on a petri dish). It "
+"enables high-resolution imaging of live tissue that may be otherwise "
+"inaccessible within the animal. The tissue is maintained alive on the "
+"imaging system through perfusion of oxygenated (95% oxygen and 5% CO2), "
+"temperature-controlled media using peristaltic pumps and microfluidics."
+msgstr ""
+
+#: ../../Glossary.md:9
+msgid "Fiji"
+msgstr ""
+
+#: ../../Glossary.md:14
+msgid ""
+"[Fiji](https://imagej.net/software/fiji/) Is Just ImageJ. ImageJ2 plus a "
+"lot of common plugins."
+msgstr ""
+
+#: ../../Glossary.md:12
+msgid "Fixation"
+msgstr ""
+
+#: ../../Glossary.md:17
+msgid ""
+"Fixation of a specimen refers to the stabilization of the "
+"cellular/molecular components within the sample while at the same time "
+"stopping any biological function in that sample. The fixative used (e.g.,"
+" paraformaldehyde, glutaraldehyde, methanol), concentration and "
+"conditions (e.g., buffer, temperature) determine the extent of "
+"preservation of the cellular and/or molecular structures within a sample,"
+" and needs to be optimized depending on the sample or structure that is "
+"being imaged."
+msgstr ""
+
+#: ../../Glossary.md:15
+msgid "Image processing"
+msgstr ""
+
+#: ../../Glossary.md:20
+msgid ""
+"Is an operation that can be performed on an image, resulting in another "
+"image. Image processing operations can be simple (e.g. resizing or "
+"rotating) or more advanced (e.g. enhancing particular features of an "
+"image like circles or lines)."
+msgstr ""
+
+#: ../../Glossary.md:18
+msgid "Immersion media"
+msgstr ""
+
+#: ../../Glossary.md:23
+msgid ""
+"The immersion media is the medium that fills the gap between your "
+"objective lens and the glass coverslip or sample. It impacts the "
+"numerical aperture of the objective lens {math}`NA=RI * sin(θ)`, thus "
+"impacting lateral and axial resolution. It is critical to match the RI of"
+" the immersion media with that of the mounting media to minimize "
+"aberrations and improve image quality. Immersion media can be air, water,"
+" silicone oil, glycerol or oil."
+msgstr ""
+
+#: ../../Glossary.md:21
+msgid "Immunolabeling"
+msgstr ""
+
+#: ../../Glossary.md:26
+msgid ""
+"Immunolabeling is one of the most common labeling techniques for fixed "
+"samples. You can use fluorescently conjugated primary antibodies to "
+"detect the protein of interest or a two-step labeling with a primary "
+"antibody and a fluorescently conjugated secondary antibody. Primary-"
+"secondary labeling tends to result in signal amplification. The main "
+"issue with immunolabeling is the size of the antibodies, which require "
+"extensive permeabilization. Another good option is to use nano-bodies, "
+"which only have the heavy-chain and are significantly smaller than "
+"regular antibodies."
+msgstr ""
+
+#: ../../Glossary.md:24
+msgid "Intravital imaging"
+msgstr ""
+
+#: ../../Glossary.md:29
+msgid ""
+"It refers to the imaging of cellular structures or biological processes "
+"inside a live animal in real time, without extracting the organs or "
+"fixing the sample. In general, it requires specific instrumentation or "
+"modalities with improved light penetration, such as multiphoton "
+"microscopy and is limited to the ability to access the specific organ, "
+"often through optical windows. Intravital imaging is overseen by "
+"bioethical committees and needs to be approved by IACUC and/or other "
+"institutional committees."
+msgstr ""
+
+#: ../../Glossary.md:27
+msgid "Mounting media"
+msgstr ""
+
+#: ../../Glossary.md:32
+msgid ""
+"Is the solution in which your specimen is placed in (mounted). Its "
+"purpose is to preserve the sample, including the fluorophores in it,  and"
+" enhance the imaging quality during acquisition, by buffering the pH, "
+"matching the refractive index throughout the sample (ideally matching it "
+"to that of glass) and minimizing photobleaching (depending on the "
+"medium). Mounting media prevents the sample from drying out allowing "
+"long-term storage."
+msgstr ""
+
+#: ../../Glossary.md:30
+msgid "Object detection"
+msgstr ""
+
+#: ../../Glossary.md:35
+msgid ""
+"Is the image processing technique to detect objects within an image. It "
+"would not give you a mask of the objects but it could give you a bounding"
+" box, or and x,y position."
+msgstr ""
+
+#: ../../Glossary.md:33
+msgid "Oxygen scavengers"
+msgstr ""
+
+#: ../../Glossary.md:38
+msgid ""
+"Oxygen tends to induce photobleaching of organic dyes and other "
+"fluorophores. Addition of oxygen scavengers to the imaging media such as "
+"glucose oxidase or pyranose 2-oxidase can significantly reduce "
+"photobleaching of the fluorophores present in the sample. It is important"
+" to understand that the use of oxygen scavengers may affect live cell "
+"imaging, as these scavengers can affect the ATP and oxygen levels within "
+"the sample, compromising its health and therefore biological function."
+msgstr ""
+
+#: ../../Glossary.md:36
+msgid "Permeabilization"
+msgstr ""
+
+#: ../../Glossary.md:41
+msgid ""
+"In order for the antibodies used during immunostaining or other "
+"fluorophores to penetrate and bind to their antigen within a cell or "
+"tissue, the membrane integrity (holes) needs to be challenged with a mild"
+" detergent. The permeabilization step needs to be carefully optimized "
+"depending on the antigen of interest, as it can result in a loss of "
+"cytoplasm or a degradation of the signal."
+msgstr ""
+
+#: ../../Glossary.md:39
+msgid "Refractive index"
+msgstr ""
+
+#: ../../Glossary.md:44
+msgid ""
+"It's a measure of how light travels through a specific medium. It is an "
+"important value when calculating the numerical aperture of an objective, "
+"Ideally, a mismatch in refractive index between the sample (mounting "
+"medium), the coverslip and immersion media should be minimized in order "
+"to enhance the image quality. [See an interactive demo of refactive index"
+" at MicroscopyU](https://www.microscopyu.com/microscopy-basics"
+"/refractive-index-index-of-refraction)"
+msgstr ""
+
+#: ../../Glossary.md:42
+msgid "ROIs"
+msgstr ""
+
+#: ../../Glossary.md:47
+msgid ""
+"Regions Of Interest. Pixels in your image that you care about (e.g., a "
+"region in tissue, a cell, a tumor, etc.)"
+msgstr ""
+
+#: ../../Glossary.md:45
+msgid "Segmentation"
+msgstr ""
+
+#: ../../Glossary.md:50
+msgid ""
+"Method of dividing an image into multiple parts or regions. There are "
+"three different types of segmentation."
+msgstr ""
+
+#: ../../Glossary.md:51
+msgid ""
+"Semantic segmentation, where all parts of an image are part of a class, "
+"common in cell biology will be detecting cells and background on an "
+"image."
+msgstr ""
+
+#: ../../Glossary.md:52
+msgid ""
+"Instance segmentation, the segmentation is object based, not just "
+"detecting were the cells are but diving each cell as a separate object."
+msgstr ""
+
+#: ../../Glossary.md:53
+msgid ""
+"Panoptic Segmentation, it can be defined as a combination of the prior "
+"two, because it identifies the object but also classifies them. An "
+"example in biology might be detecting all the cells on an image and "
+"classifying them as dividing vs not."
+msgstr ""
+
+#: ../../Glossary.md:51
+msgid "Thresholding"
+msgstr ""
+
+#: ../../Glossary.md:56
+msgid ""
+"The easiest form of image segmentation, it divides the image into two "
+"part the background and the foreground (or signal). It creates a binary "
+"image where usually the background pixels would be change to a 0 value "
+"and the foreground pixels values would be 1."
+msgstr ""
+
+#: ../../Glossary.md:54
+msgid "Tissue clearing"
+msgstr ""
+
+#: ../../Glossary.md:59
+msgid ""
+"Fluorescence imaging of the whole thickness of a piece of tissue is very "
+"challenging due to light absorption and scattering induced by the "
+"inhomogeneities in refractive indexes within the tissue itself, resulting"
+" in poor light penetration. Additionally, light coming from different "
+"parts of the sample contribute to fluorescence blur, drastically reducing"
+" contrast and resolution in any given plane. As a result, researchers "
+"tend to use tissue sectioning techniques to extract information about "
+"cellular components and their spatial distribution or relationships from "
+"a thin two-dimensional volume. However, most components in any complex "
+"biological system such as an organ are not contained within this two-"
+"dimensional volume, and therefore, this approach compromises the "
+"understanding of the spatial relationships among cellular components. "
+"Tissue clearing focused on reducing the inhomogeneities in the tissue by "
+"equilibrating the refractive index throughout the sample. This allows "
+"light to pass through the tissue and therefore enables high resolution, "
+"volumetric imaging of whole organs and tissues using conventional "
+"microscopy techniques such as confocal microscopy without the need to "
+"physically section the sample."
+msgstr ""
+
+#: ../../Glossary.md:57
+msgid "Tissue sectioning"
+msgstr ""
+
+#: ../../Glossary.md:62
+msgid ""
+"Light penetration and fluorescence imaging is negatively impacted by "
+"light scattering within a thick specimen. This scattering is due to the "
+"different refractive indexes present within a tissue. To facilitate "
+"imaging of tissues, researchers often cut thick tissues into slices of "
+"different thicknesses. This process is called tissue sectioning. In most "
+"cases the samples are fixed and embedded in paraffin or frozen in tissue "
+"freezing medium and later cut into thin slices by a machine like a "
+"cryostat, microtome, or vibratome and sections collected into a tube or "
+"onto a slide."
+msgstr ""
+