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Nikita Vladimirov edited this page Aug 20, 2024 · 7 revisions

Room requirements

These are similar to the mesoSPIM-V5, except the optical table requirement.

  • The floor should be solid concrete to avoid vibrations from walking.

  • Windows should be covered with black curtains or absent.

  • Enough space for a desk with a large (40inch) screen for the imaging computer and 2-3 chairs.

  • Power outlets: Foresee enough power outlets for the instrument. Total power uptake is a few kW at maximum

  • Optical table is not required. The Benchtop mesoSPIM, as its name suggests, can be placed on a sturdy bench, desk, or even a cart.

  • Active ventilation is required when working with BABB, DBE, or any other toxic chemicals.

  • Air conditioning, especially for long-running acquisitions.

  • For sample preparation, a wet lab bench, a hood and a sink in the vicinity of the microscope.

Safety ⚠️

  • Chemicals must be handled in a fume hood, and stored in a safe cabinet (many of them are toxic and/or highly flammable).

  • Observe all relevant chemical, laser, trash disposal, and fire hazard safety rules of your institute. Take the safety rules seriously, they are written in blood.

  • Do not dispose chemicals into the water stream.

Examples of ventilation duct arrangement, sink and hume hood are shown below.

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