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Best practices in processing EM data

Michael A. Cianfrocco edited this page Jun 7, 2018 · 1 revision

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##Best practices in processing EM data

As a maturing field of structural biology, analysis of EM particle images requires a trained eye in order to prevent model overfitting and appropriate analysis techniques. Below is a list of important topics related to best practices that users can follow to avoid any potential pitfalls.

  1. Model bias (or, how do I know if my 3D structure is correct?

  2. Picking a box size & pixel size for particle processing

  3. Particle normalization

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