Manual particle picking

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Picking particles

Picking individual 'single particles' from your micrograph images requires

Guidelines for picking particles

Make sure to read through particle picking guidelines to maximize the effectiveness of your data processing.

Particle picking approaches

Which method should I use to pick my particles? Manual or automatic? Please read here for information related to how to approach picking your data.

Manual picking:

• EMAN2: e2boxer.py
• EMAN: boxer

Semi-automatic picking:

• EMAN2: SWARM in e2boxer.py

Automatic picking:

• Signature

Tilt pair picking: (utilized for RCT/OTR data analysis)

• Manual
• Semi-automated